Research Areas

Our research focuses on determining the molecular functions of human cytomegalovirus (HCMV) proteins during infection and disease. HCMV is a member of the beta-herpesvirus family of viruses which includes HHV-6 and 7. Infection occurs upon exposure to virus-containing body fluids, is life-long and generally asymptomatic in healthy children and adults. However, during pregnancy, HCMV infection may result in congenital birth defects including hearing loss and neurological damage. In immunologically immature or compromised children and adults, infection often results in life threatening diseases. And, increasing evidence suggestions that persistent life-long HCMV infection is associated with numerous chronic diseases including atherosclerosis, immuno-senescence, cancer and possibly Alzheimer’s Disease.

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Current Members

Christopher Monti

G3 Student

cmonti@mcw.edu

Suzette Rosas

Graduate Student

srosas@mcw.edu

Critical Role for the Human Cytomegalovirus Major Immediate Early Proteins in Recruitment of RNA Polymerase II and H3K27Ac To an Enhancer-Like Element in OriLyt.

(Forte E, Li M, Ayaloglu Butun F, Hu Q, Borst EM, Schipma MJ, Piunti A, Shilatifard A, Terhune SS, Abecassis M, Meier JL, Hummel M.) Microbiol Spectr. 2023 Feb 14;11(1):e0314422 PMID: 36645269 PMCID: PMC9927211 SCOPUS ID: 2-s2.0-85148092420 01/17/2023
Computational modeling of protracted HCMV replication using genome substrates and protein temporal profiles.

(Monti CE, Mokry RL, Schumacher ML, Dash RK, Terhune SS.) Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201787119 PMID: 35994667 PMCID: PMC9437303 SCOPUS ID: 2-s2.0-85136192098 08/23/2022
Nitric Oxide Attenuates Human Cytomegalovirus Infection yet Disrupts Neural Cell Differentiation and Tissue Organization.

(Mokry RL, O'Brien BS, Adelman JW, Rosas S, Schumacher ML, Ebert AD, Terhune SS.) J Virol. 2022 Jul 27;96(14):e0012622 PMID: 35862705 PMCID: PMC9327702 SCOPUS ID: 2-s2.0-85135227929 07/22/2022
Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus.

(O'Brien BS, Mokry RL, Schumacher ML, Pulakanti K, Rao S, Terhune SS, Ebert AD.) iScience. 2022 Apr 15;25(4):104098 PMID: 35391828 PMCID: PMC8980761 04/09/2022
Human cytomegalovirus lytic infection inhibits replication-dependent histone synthesis and requires stem loop binding protein function.

(Albright ER, Morrison K, Ranganathan P, Carter DM, Nishikiori M, Lee JH, Slayton MD, Ahlquist P, Terhune SS, Kalejta RF.) Proc Natl Acad Sci U S A. 2022 Apr 05;119(14):e2122174119 PMID: 35344424 PMCID: PMC9169081 SCOPUS ID: 2-s2.0-85127264685 03/29/2022
Method to Study Adaptive NK Cells Following MCMV Infections.

(Khalil M, Mei A, Hashemi E, Wang D, Schumacher M, Terhune S, Malarkannan S.) Methods Mol Biol. 2022;2463:195-204 PMID: 35344176 SCOPUS ID: 2-s2.0-85127680170 03/29/2022
Crosstalk between Plk1, p53, cell cycle, and G2/M DNA damage checkpoint regulation in cancer: computational modeling and analysis.

(Jung Y, Kraikivski P, Shafiekhani S, Terhune SS, Dash RK.) NPJ Syst Biol Appl. 2021 Dec 09;7(1):46 PMID: 34887439 PMCID: PMC8660825 SCOPUS ID: 2-s2.0-85121043614 12/11/2021
MyD88 is an essential regulator of NK cell-mediated clearance of MCMV infection.

(Dixon KJ, Siebert JR, Wang D, Abel AM, Johnson KE, Riese MJ, Terhune SS, Tarakanova VL, Thakar MS, Malarkannan S.) Mol Immunol. 2021 Sep;137:94-104 PMID: 34242922 SCOPUS ID: 2-s2.0-85109126048 07/10/2021
Implications of a 'Third Signal' in NK Cells.

(Khalil M, Wang D, Hashemi E, Terhune SS, Malarkannan S.) Cells. 2021 Jul 31;10(8) PMID: 34440725 PMCID: PMC8393955 SCOPUS ID: 2-s2.0-85115044374 08/28/2021
Nitric Oxide Circumvents Virus-Mediated Metabolic Regulation during Human Cytomegalovirus Infection.

(Mokry RL, Schumacher ML, Hogg N, Terhune SS.) mBio. 2020 Dec 15;11(6) PMID: 33323506 PMCID: PMC7773989 SCOPUS ID: 2-s2.0-85098068781 12/17/2020
Prospects for Clinical Development of Stat5 Inhibitor IST5-002: High Transcriptomic Specificity in Prostate Cancer and Low Toxicity In Vivo.

(Maranto C, Udhane V, Jia J, Verma R, Müller-Newen G, LaViolette PS, Pereckas M, Sabharwal L, Terhune S, Pattabiraman N, Njar VCO, Imig JD, Wang L, Nevalainen MT.) Cancers (Basel). 2020 Nov 18;12(11) PMID: 33217941 PMCID: PMC7724566 SCOPUS ID: 2-s2.0-85096199725 11/22/2020
Network mechanisms and dysfunction within an integrated computational model of progression through mitosis in the human cell cycle.

(Terhune SS, Jung Y, Cataldo KM, Dash RK.) PLoS Comput Biol. 2020 Apr;16(4):e1007733 PMID: 32251461 PMCID: PMC7162553 SCOPUS ID: 2-s2.0-85083620688 04/07/2020
Erratum for Rak et al., Human Cytomegalovirus UL135 Interacts with Host Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency.

(Rak MA, Buehler J, Zeltzer S, Reitsma J, Molina B, Terhune S, Goodrum F.) J Virol. 2020 Mar 17;94(7) PMID: 32184335 PMCID: PMC7081901 SCOPUS ID: 2-s2.0-85082001595 03/19/2020
Nitric oxide circumvents virus-mediated metabolic regulation during human cytomegalovirus infection

(Mokry RL, Schumacher ML, Hogg N, Terhune SS.) mBio. November-December 2020;11(6):1-25 SCOPUS ID: 2-s2.0-85098068781 11/01/2020
Human Cytomegalovirus Disruption of Calcium Signaling in Neural Progenitor Cells and Organoids.

(Sison SL, O'Brien BS, Johnson AJ, Seminary ER, Terhune SS, Ebert AD.) J Virol. 2019 Sep 01;93(17) PMID: 31217241 PMCID: PMC6694809 SCOPUS ID: 2-s2.0-85071355135 06/21/2019
Human Cytomegalovirus UL135 Interacts with Host Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency.

(Rak MA, Buehler J, Zeltzer S, Reitsma J, Molina B, Terhune S, Goodrum F.) J Virol. 2018 Oct 15;92(20) PMID: 30089695 PMCID: PMC6158428 SCOPUS ID: 2-s2.0-85052151483 08/10/2018
Tumor Necrosis Factor Alpha Induces Reactivation of Human Cytomegalovirus Independently of Myeloid Cell Differentiation following Posttranscriptional Establishment of Latency.

(Forte E, Swaminathan S, Schroeder MW, Kim JY, Terhune SS, Hummel M.) mBio. 2018 Sep 11;9(5) PMID: 30206173 PMCID: PMC6134100 SCOPUS ID: 2-s2.0-85055538738 09/13/2018
Isomer-specific effect of microRNA miR-29b on nuclear morphology.

(Kriegel AJ, Terhune SS, Greene AS, Noon KR, Pereckas MS, Liang M.) J Biol Chem. 2018 Sep 07;293(36):14080-14088 PMID: 30006350 PMCID: PMC6130969 SCOPUS ID: 2-s2.0-85053013199 07/15/2018
Association of Mycobacterium Proteins with Lipid Droplets.

(Armstrong RM, Carter DC, Atkinson SN, Terhune SS, Zahrt TC.) J Bacteriol. 2018 Aug 15;200(16) PMID: 29760207 PMCID: PMC6060360 SCOPUS ID: 2-s2.0-85050494395 05/16/2018
Impact of RNA polymerase I inhibitor CX-5461 on viral kinase-dependent and -independent cytomegalovirus replication.

(Westdorp KN, Terhune SS.) Antiviral Res. 2018 May;153:33-38 PMID: 29458130 PMCID: PMC6015744 SCOPUS ID: 2-s2.0-85043578832 02/20/2018
Correction for Mounce et al., A Conserved Gammaherpesvirus Protein Kinase Targets Histone Deacetylases 1 and 2 To Facilitate Viral Replication in Primary Macrophages.

(Mounce BC, Mboko WP, Bigley TM, Terhune SS, Tarakanova VL.) J Virol. 2018 Jan 01;92(1) PMID: 29242252 PMCID: PMC5730793 SCOPUS ID: 2-s2.0-85037989790 12/16/2017
SmgGDS is a transient nucleolar protein that protects cells from nucleolar stress and promotes the cell cycle by regulating DREAM complex gene expression.

(Gonyo P, Bergom C, Brandt AC, Tsaih SW, Sun Y, Bigley TM, Lorimer EL, Terhune SS, Rui H, Flister MJ, Long RM, Williams CL.) Oncogene. 2017 Dec 14;36(50):6873-6883 PMID: 28806394 PMCID: PMC5730474 SCOPUS ID: 2-s2.0-85038242723 08/15/2017
Cytomegalovirus Late Protein pUL31 Alters Pre-rRNA Expression and Nuclear Organization during Infection.

(Westdorp KN, Sand A, Moorman NJ, Terhune SS.) J Virol. 2017 Sep 15;91(18) PMID: 28659485 PMCID: PMC5571270 SCOPUS ID: 2-s2.0-85028302530 07/01/2017
Proteomic Screen for Cellular Targets of the Vaccinia Virus F10 Protein Kinase Reveals that Phosphorylation of mDia Regulates Stress Fiber Formation.

(Greseth MD, Carter DC, Terhune SS, Traktman P.) Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S124-S143 PMID: 28183815 PMCID: PMC5393388 SCOPUS ID: 2-s2.0-85017129288 02/12/2017
Opposing Regulation of the EGF Receptor: A Molecular Switch Controlling Cytomegalovirus Latency and Replication.

(Buehler J, Zeltzer S, Reitsma J, Petrucelli A, Umashankar M, Rak M, Zagallo P, Schroeder J, Terhune S, Goodrum F.) PLoS Pathog. 2016 May;12(5):e1005655 PMID: 27218650 PMCID: PMC4878804 SCOPUS ID: 2-s2.0-84973327135 05/25/2016

Terhune Laboratory