Riehle Laboratory

Location

Microbiology & Immunology
BSB B2920

General Interests

Malaria Vector Biology, Genetics of Susceptibility, Microbiome and Immunity

Research Areas

Malaria remains a major global health problem leading to significant human morbidity and mortality. The World Health Organization reports that about half a million people die of malaria each year with most of these deaths occurring in children in Sub-Saharan Africa. Significant gains in malaria control have been made in recent years largely on the backs of concerted vector control efforts including the use of bed nets and insecticides. Our area of research focuses on understanding the dynamics of the relationship between the mosquito vector and the malaria parasite, Plasmodium falciparum. Through a better understanding of this relationship we can inform the design of future control strategies aimed at malaria eradication.

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Current Members

Elise Farley

Research Technologist I

efarley@mcw.edu

Heather L. Eggleston, PhD

Postdoctoral Fellow

heggleston@mcw.edu

Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus

(Grau-Bové X, Lucas E, Pipini D, Rippon E, van ‘t Hof AE, Constant E, Dadzie S, Egyir-Yawson A, Essandoh J, Chabi J, Djogbénou L, Harding NJ, Miles A, Kwiatkowski D, Donnelly MJ, Weetman D, Amaya-Romero JE, Ayala D, Battey CJ, Bejon P, Besansky NJ, Burt A, Cano J, Caputo B, Constant E, Costantini C, Coulibaly B, della Torre A, Diabaté A, Dinis J, Donnelly MJ, Drury E, Edouardo J, Elissa N, Essandoh J, Fontaine MC, Godfray CHJ, Hahn MW, Harding NJ, Henrichs C, Hubbart C, Isaacs AT, Jawara M, Jeffreys AE, Jyothi D, Kamali M, Kern AD, Kwiatkowski DP, Clarkson CS, Lawniczak MKN, Le Goff G, Lucas ER, Malangone C, Mawejje HD, Mbogo C, Mead D, Midega J, Miles A, Nwakanma DC, O’Loughlin S, Pinto J, Riehle MM, Robert V, Rockett KA, Rohatgi KR, Rowlands K, Schrider DR, Sharakhov I, Simpson V, Stalker J, Troco AD, Vernick KD, Weetman D, Bradley J.) PLoS Genetics. 21 January 2021;17(1) SCOPUS ID: 2-s2.0-85099805834 01/21/2021
Genome variation and population structure among 1142 mosquitoes of the African malaria vector species Anopheles gambiae and Anopheles coluzzii.

(Anopheles gambiae 1000 Genomes Consortium.) Genome Res. 2020 10;30(10):1533-1546 PMID: 32989001 PMCID: PMC7605271 SCOPUS ID: 2-s2.0-85092682445 09/30/2020
Exposure of Anopheles mosquitoes to trypanosomes reduces reproductive fitness and enhances susceptibility to Plasmodium.

(Dieme C, Zmarlak NM, Brito-Fravallo E, Travaillé C, Pain A, Cherrier F, Genève C, Calvo-Alvarez E, Riehle MM, Vernick KD, Rotureau B, Mitri C.) PLoS Negl Trop Dis. 2020 02;14(2):e0008059 PMID: 32032359 PMCID: PMC7032731 SCOPUS ID: 2-s2.0-85081144453 02/08/2020
Gene copy number and function of the APL1 immune factor changed during Anopheles evolution.

(Mitri C, Bischoff E, Eiglmeier K, Holm I, Dieme C, Brito-Fravallo E, Raz A, Zakeri S, Nejad MIK, Djadid ND, Vernick KD, Riehle MM.) Parasit Vectors. 2020 Jan 13;13(1):18 PMID: 31931885 PMCID: PMC6958605 SCOPUS ID: 2-s2.0-85077786231 01/15/2020
Leucine-Rich Immune Factor APL1 Is Associated With Specific Modulation of Enteric Microbiome Taxa in the Asian Malaria Mosquito Anopheles stephensi.

(Mitri C, Bischoff E, Belda Cuesta E, Volant S, Ghozlane A, Eiglmeier K, Holm I, Dieme C, Brito-Fravallo E, Guelbeogo WM, Sagnon N, Riehle MM, Vernick KD.) Front Microbiol. 2020;11:306 PMID: 32174902 PMCID: PMC7054466 03/17/2020
Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii.

(Nardini L, Holm I, Pain A, Bischoff E, Gohl DM, Zongo S, Guelbeogo WM, Sagnon N, Vernick KD, Riehle MM.) Sci Rep. 2019 10 24;9(1):15275 PMID: 31649293 PMCID: PMC6813320 SCOPUS ID: 2-s2.0-85074074218 10/28/2019
Genetic diversity of the African malaria vector Anopheles gambiae.

(Anopheles gambiae 1000 Genomes Consortium, Data analysis group, Partner working group, Sample collections—Angola:, Burkina Faso:, Cameroon:, Gabon:, Guinea:, Guinea-Bissau:, Kenya:, Uganda:, Crosses:, Sequencing and data production, Web application development, Project coordination.) Nature. 2017 12 07;552(7683):96-100 PMID: 29186111 PMCID: PMC6026373 SCOPUS ID: 2-s2.0-85038811995 12/01/2017
The Anopheles gambiae 2La chromosome inversion is associated with susceptibility to Plasmodium falciparum in Africa.

(Riehle MM, Bukhari T, Gneme A, Guelbeogo WM, Coulibaly B, Fofana A, Pain A, Bischoff E, Renaud F, Beavogui AH, Traore SF, Sagnon N, Vernick KD.) Elife. 2017 06 23;6 PMID: 28643631 PMCID: PMC5482571 SCOPUS ID: 2-s2.0-85021397890 06/24/2017
Preferential suppression of Anopheles gambiae host sequences allows detection of the mosquito eukaryotic microbiome.

(Belda E, Coulibaly B, Fofana A, Beavogui AH, Traore SF, Gohl DM, Vernick KD, Riehle MM.) Sci Rep. 2017 06 12;7(1):3241 PMID: 28607435 PMCID: PMC5468309 SCOPUS ID: 2-s2.0-85020718351 06/14/2017
Correction: An Evolution-Based Screen for Genetic Differentiation between Anopheles Sister Taxa Enriches for Detection of Functional Immune Factors.

(Mitri C, Bischoff E, Takashima E, Williams M, Eiglmeier K, Pain A, Guelbeogo WM, Gneme A, Brito-Fravallo E, Holm I, Lavazec C, Sagnon N, Baxter RH, Riehle MM, Vernick KD.) PLoS Pathog. 2016 Aug;12(8):e1005836 PMID: 27518517 PMCID: PMC4982601 08/16/2016
Malaria vector populations across ecological zones in Guinea Conakry and Mali, West Africa.

(Coulibaly B, Kone R, Barry MS, Emerson B, Coulibaly MB, Niare O, Beavogui AH, Traore SF, Vernick KD, Riehle MM.) Malar J. 2016 Apr 08;15:191 PMID: 27059057 PMCID: PMC4826509 SCOPUS ID: 2-s2.0-84966415575 04/10/2016
Evolution of GOUNDRY, a cryptic subgroup of Anopheles gambiae s.l., and its impact on susceptibility to Plasmodium infection.

(Crawford JE, Riehle MM, Markianos K, Bischoff E, Guelbeogo WM, Gneme A, Sagnon N, Vernick KD, Nielsen R, Lazzaro BP.) Mol Ecol. 2016 Apr;25(7):1494-510 PMID: 26846876 SCOPUS ID: 2-s2.0-84960921872 02/06/2016

Microbiology & Immunology